Does CalibCurve replace a LIMS?

No, and it is not intended to. A LIMS manages samples, workflows and results across an entire laboratory and typically costs from around €5,000 per year. CalibCurve does the calibration part only, as a one-time purchase of €399. Many laboratories need rigorous calibration documentation without the cost and implementation effort of a full LIMS.

Does CalibCurve require an internet connection?

No. CalibCurve runs entirely offline. No internet connection is required to install, activate or use it, which makes it suitable for isolated and validated laboratory networks. No data leaves the machine.

Is CalibCurve a subscription?

No. CalibCurve is a one-time purchase of €399 for a perpetual licence per installation. There is no annual fee and no maintenance contract. Future major versions are optional paid upgrades and are never a condition of continuing to use the version you bought.

Analytical chemistry · Windows desktop

Calibration you can defend in an audit.

Q: What is CalibCurve used for?

CalibCurve creates, evaluates and documents calibration curves for analytical instruments. It replaces spreadsheet-based calibration with validated regression, statistics computed to recognised standards, enforced acceptance criteria and an audit trail. It is used in pharmaceutical, environmental, food, clinical, industrial and academic laboratories.

Q: Which regression models does CalibCurve support?

Unweighted linear regression, weighted 1/x and weighted 1/x² regression for methods where variance scales with concentration such as LC-MS/MS, and four-parameter logistic (4PL) regression for immunoassay and ELISA work.

Q: How are LOD and LOQ calculated?

Limit of detection and limit of quantitation are calculated according to DIN 32645, using 3.3 times the residual standard deviation divided by the slope for LOD, and 10 times the residual standard deviation divided by the slope for LOQ.

Q: Is CalibCurve ISO 17025 certified?

Software cannot itself be ISO 17025 accredited — accreditation applies to the laboratory. CalibCurve is designed to support ISO 17025 documentation practice through its audit trail, analyst attribution, enforced acceptance criteria and standardised reporting. Validation for a specific regulated use remains the responsibility of the laboratory.

Q: Does the software decide whether a calibration passes?

No. CalibCurve computes the statistics and proposes a status, and it labels that proposal explicitly as a suggestion. The analyst writes and owns the final interpretation, which appears in the report attributed to them by name.

CalibCurve replaces the spreadsheet your calibration currently lives in. Validated regression, statistics computed to standard, acceptance criteria enforced rather than remembered, and a complete audit trail — on your own machine, with no internet connection required.

€399 one-time · perpetual licence · per installation

Order a licence What it does

Comparable capability in a LIMS starts around €5,000 per year. CalibCurve does the calibration part, once, for €399 — because most laboratories do not need the other ninety percent of a LIMS.

CalibCurve calibration workflow: five guided steps with regression results and live preview chart

The problem

Everyone knows the spreadsheet is a liability. Nobody has time to replace it.

Calibration in Excel works right up until somebody asks a question about it. Who changed this cell? Which points were excluded, and on what grounds? Is that LOD computed the way the method says? Was this curve ever actually accepted, or did it just get used?

Formulas nobody owns

The workbook was built by someone who left in 2019. It has been copied eleven times. Two of the copies have a different LOD formula, and no one is certain which is right.

Silent exclusions

A point gets deleted because it "looked wrong". There is no record that it existed, no reason attached, and no statistical test that justified removing it.

Documentation after the fact

The report is assembled by hand at the end, from numbers copied out of cells. Every transcription is an opportunity for an error that nothing will catch.

What CalibCurve does

Five steps, and the statistics are not optional.

Method, data, calculation, review, export. The workflow is deliberately linear because calibration is a procedure, not an exploration.

Regression that matches the method

Unweighted linear for standard work. Weighted 1/x and 1/x² where variance scales with concentration — LC-MS/MS, wide dynamic ranges. Four-parameter logistic for immunoassay and ELISA, where a straight line was never the right model.

Slope, intercept and regression equation with 95% confidence intervals
Pearson r and coefficient of determination R²
Standard error of slope and intercept, Student's t critical values
LOD and LOQ computed to DIN 32645 — 3.3·s/m and 10·s/m

Regression calculation step with live preview and R², LOD and LOQ results

Diagnostics that find the point you should have questioned

A high R² hides a great deal. CalibCurve runs the diagnostics that actually detect a bad calibration: residual patterns, leverage, and influence — so a single point quietly bending the whole curve is flagged rather than averaged away.

Residual plot with ±2σ bands and non-random pattern detection
Cook's distance, DFBETA and leverage per calibration point
Influence diagram — point status shown as Normal, High residual, High leverage or High influence
Full ANOVA table: sums of squares, degrees of freedom, mean squares, F-statistic, p-value
Response factor plot with ±20% acceptance band

Residual plot, ANOVA and influence diagnostics with Cook's D and DFBETA per point

Acceptance criteria the software enforces

QC levels are defined with the method, not decided afterwards. Low, mid and high controls are back-calculated against the curve, recovery and bias are computed, and the result is a pass or a fail against tolerances that were set in advance.

Automatic warnings: R² below threshold, F-test failure, non-random residuals, outliers beyond 2σ, intercept significantly non-zero, recovery outside limits
Configurable tolerances — typically ±10–15% at LLOQ and ULOQ, ±5% mid-range
Sample concentration calculator with back-calculation and recovery

CalibCurve dashboard with audit trail listing calibrations, methods, analytes and analysts

The software proposes. The analyst decides.

This is the part that matters for accreditation, and it is a deliberate design decision. CalibCurve will suggest a calibration status and offer wording for the QC summary — and it labels both, explicitly, as suggestions. The conclusion that goes into the report is written by the analyst, who is named in it.

Report builder with selectable sections — core, extended, assessment
Suggested status (Passed / Warning / Failed) shown as a proposal, never applied silently
Free-text analyst conclusion, required and attributed
Short report or full report, exported as PDF

Report builder with section selection and explicit wording that the analyst decides the interpretation

Method templates

Your technique is already in there.

Templates carry the regression model, units, QC levels and acceptance criteria for a technique, so a routine calibration starts configured rather than blank.

HPLC / UV

Paracetamol active-substance assay, caffeine in beverages, vitamin C. Linear and weighted models.

GC

GC-FID and GC-MS: ethanol in extracts, methanol in production samples, acetone in air samples.

ICP & AAS

ICP-MS copper in drinking water, ICP-OES zinc in soil, AAS iron in alloys.

UV-VIS photometry

Nitrate and phosphate determination, BCA and Bradford protein assays.

Bioanalytics

ELISA protein quantification with 4PL, enzyme activity, qPCR DNA copy number.

Titration & sensors

pH electrode and conductivity calibration, ISE fluoride, Ca/Mg hardness, TOC in water.

Who it is for

Laboratories that report results somebody relies on.

Pharmaceutical

Drug substance assays, dissolution testing, stability studies. QC and QA departments.

Environmental

Water quality — heavy metals and anions — soil contamination, air quality monitoring.

Food & beverage

Nutritional analysis, contaminant testing, routine quality control.

Clinical

Therapeutic drug monitoring and biomarker quantification.

Contract research

CROs and contract testing laboratories running many methods across many clients.

Academic & R&D

University laboratories and method development groups.

Specifications

Technical details

Platform	Windows 10 and 11, 64-bit. Desktop application — no browser, no server component.
Installation	Signed installer. Installs to a normal user profile; administrator rights not required for standard use.
Connectivity	Fully offline. No internet connection required at any point, including activation. Suitable for isolated and validated laboratory networks.
Languages	German and English, switchable in the application.
Regression models	Unweighted linear · weighted 1/x · weighted 1/x² · four-parameter logistic (4PL)
Statistics	R², Pearson r, 95% confidence intervals, standard errors, full ANOVA, LOD/LOQ to DIN 32645, residual analysis, Cook's D, DFBETA, leverage
Data entry	Spreadsheet-style table with paste from Excel. Up to three replicates per level with automatic CV%.
Data storage	Local database. Calibrations, methods, analysts and instruments retained with timestamps.
Audit trail	Complete calibration history with analyst attribution, filterable and exportable to CSV.
Reporting	Configurable PDF reports — short or full — with embedded plots and a named analyst conclusion.
Compliance	Designed to support ISO 17025 documentation practice. LOD/LOQ per DIN 32645.
Licence	One-time purchase, perpetual, per installation. No subscription and no recurring fee.

A note on compliance wording: CalibCurve is built to support documentation practice under ISO 17025 and computes LOD and LOQ according to DIN 32645. It is a calculation and documentation tool. Accreditation belongs to the laboratory, and validation for a specific regulated use remains the laboratory's responsibility.

Questions

Frequently asked

What is CalibCurve used for?

It creates, evaluates and documents calibration curves for analytical instruments — replacing spreadsheet calibration with validated regression, standard statistics, enforced acceptance criteria and an audit trail. Used in pharmaceutical, environmental, food, clinical, industrial and academic laboratories.

Does it replace a LIMS?

No, and it is not meant to. A LIMS manages samples and workflows across a whole laboratory and starts around €5,000 per year. CalibCurve does the calibration part only, once, for €399. Many laboratories need rigorous calibration documentation without a LIMS implementation.

Which regression models are supported?

Unweighted linear, weighted 1/x and 1/x² for methods where variance scales with concentration such as LC-MS/MS, and four-parameter logistic (4PL) for immunoassay and ELISA.

How are LOD and LOQ calculated?

According to DIN 32645 — 3.3·s/m for the limit of detection and 10·s/m for the limit of quantitation, where s is the residual standard deviation and m the slope.

Is CalibCurve ISO 17025 certified?

Software cannot be ISO 17025 accredited; accreditation applies to the laboratory. CalibCurve is designed to support ISO 17025 documentation practice through its audit trail, analyst attribution, enforced acceptance criteria and standardised reporting. Validation for a specific regulated use remains the laboratory's responsibility.

Does it need an internet connection?

No. CalibCurve runs entirely offline — no connection is required to install, activate or use it. Suitable for isolated and validated laboratory networks. No data leaves the machine.

Is it a subscription?

No. One-time purchase of €399 for a perpetual licence per installation. No annual fee, no maintenance contract. Future major versions are optional paid upgrades, never a condition of continuing to use what you bought.

Does the software decide whether a calibration passes?

No. It computes the statistics and proposes a status — explicitly labelled as a suggestion. The analyst writes and owns the final interpretation, and is named in the report.